EnviroDEFENSE® PurPond Aquaculture

EnviroDEFENSE® PurPond Aquaculture is designed for use in fish and prawn ponds, lakes and lagoons where organic odors and organic accumulations are a problem. Contains naturally occurring bacteria and enzymes, which have been selected and produced for their ability to break down organic matter and reduce ammonia accumulations through a natural oxidative process. EnviroDEFENSE® PurPond Aquaculture digests these organics naturally and safely and keeps pond water fresh and disease free.

PRODUCT SPECIFICATIONS: In addition to 4 strains of bacteria (Bacillus amyloliquefaciens, Bacillus pumilus, Bacillus licheniformis, Bacillus megaterium), EnviroDEFENSE® PurPond contains the following enzymes for rapid degradation of waste:

• Protease: Digestion of Protein.
• Amylase: Digestion of Starch.
• Cellulase: Digestion of Cellulosic Particles.
• Lipase: Digestion of Fats & Oils.

APPLICATIONS:
Pond/Water Garden Application: Apply 1 oz. (29.57 ml) per every 1,000 gallons (3.78 L) of pond water.

Aquarium Application:
Start Up Application: Apply ½ Teaspoon (2.46 ml) per every 500 Gallons (1,893 L).
Routine Application: Apply ¼ Teaspoon (1.23 ml) per every 500 Gallons (1,893 L).

PARAMETERS: For biology to work properly in the pond, certain water conditions must exist:

• pH of the water maintained between 5.5 & 9.5.
• Temperature of the water above 10°C (50°F).
• Dissolved oxygen levels must be above 3 ppm.
• Water must be free of chlorine/chemicals that will kill beneficial bacteria.

EnviroDEFENSE® PurPond Aquaculture Studies
In 1997, a comprehensive study was performed in the Philippines and Thailand at various shrimp farms to test the efficacy of EnviroDEFENSE® PurPond against regional competitors. The white paper presentation was delivered at the 1997 World Aquaculture Conference in Seattle, WA – Title: Efficacy of the Three Brands of Probiotics in Enhancing and Maintaining Optimum Physio-Chemical and Biological Parameters in Ponds Stocked with Black Tiger Shrimp at 40 OSM. (The testing was overseen by an official with SEAFDEC).

Research Objectives

• To determine which among the three brands of probiotics was the most effective at enhancing and maintaining optimum physio-chemical and biological parameters of shrimp rearing environments.
• To identify which of the three probiotic brands was the most economical to use in culture operations.
• To develop the most appropriate method of administering probiotic materials in ponds.
• To be able to incorporate efficient use of probiotics into the research company’s protocol.

Mindanao, Philippines Test Results (Highlights) – Full Data Set Available Upon Request
Results after 149 day grow out period and weekly treatments with PurPond

• Considerably higher survival rates than competitors – 90% Survival Rate
• Survival Rates of Competitor 1 and 2: 45% and 23% Respectively
• Ponds were not hit by “one month disease syndrome”
• Product was 2 times the amount of Competitor 1 and 3

Phuket Island, Thailand Test Results (Highlights) – Full Data Set Available Upon Request
Results after 149 day grow out period and weekly treatments with PurPond

• Considerably higher survival rates than competitors – 85% Survival Rate
• Ponds were not hit by “one month disease syndrome”
• Production: Shrimp experienced an average length of 10 cm at mid-cycle which would normally take 10-11 weeks

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